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. 2014 Mar 18;9(3):e92252. doi: 10.1371/journal.pone.0092252

Figure 4. Effect of EF on the expression of MMP-2 and TIMP-2.

Figure 4

EF stimulation enhanced the expression of MMP-2 (A), but not its endogenous inhibiting molecule TIMP-2 (B). Cell lysates were prepared after treatment of HTR-8/SVneo cells with EFs (150 mV/mm) for 5 and 10 h, respectively, and Western blot was done for the expression of MMP-2 and TIMP-2 as mentioned in Materials and Methods. A and B are representative of MMP-2 and TIMP-2 protein signal respectively, and house-keeping gene GAPDH as internal controls. Histograms depicting respective MMP-2 and TIMP-2 protein levels normalized to GAPDH. The error bars represent the S.E. (*p<0.05).