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. 2004 Apr 15;18(8):952–963. doi: 10.1101/gad.1174704

Figure 2.

Figure 2.

Skeletal development in Runx3–/– and Runx2+/–3–/– mice. (A–C) Hind limb skeletons of wild-type (A), Runx3–/– (B), and Runx2+/–3–/– (C) newborns. Calcified tissues were stained red with Alizarin red, and cartilage was stained blue with Alcian blue. Wild-type and Runx3–/– limb skeletons showed a similar pattern of mineralization (A,B), whereas the mineralized regions of Runx2+/–3–/– limbs were much smaller (C). (D–F) Histological analysis of the tibiae of wild-type (D), Runx3–/– (E), and Runx2+/–3–/– (F) embryos at E18.5. The sections were stained with H&E and Kossa. (G–O) In situ hybridization using the antisense probes of Col2a1 (G–I), Col10a1 (J–L), and osteopontin (M–O). We detected no signal using sense probes of Col2a1, Col10a1, or osteopontin (data not shown). In the tibiae of wild-type and Runx3–/– embryos, the cartilage was largely replaced by bone (D,E), osteopontin-positive osteoblasts occupied the bone marrow (M,N), and Col2a1-positive (G,H) or Col10a1-positive (J,K) chondrocytes were observed in the epiphyses. Runx2+/–3–/– tibiae were still cartilaginous (F), and the chondrocytes expressed Col2a1 (I) or Col10a1 (L) except for those in the mineralized diaphyses, which were occupied by osteopontin-positive terminal hypertrophic chondrocytes (O). Bars: A–C, 1 mm; D–O, 500 μm.