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. 2013 Nov 21;60(1):37–46. doi: 10.1262/jrd.2013-083

Fig. 1.

Fig. 1.

Expression of Fucci transgenes in the undifferentiated spermatogonia compartment. A: Histological sections of Cdt1-KO2 and Gem-AG transgenic mouse testes. Cdt1-KO2 is expressed predominantly in cells on the basement membrane, while Gem-AG expression is rarely found in the same region. B: Immunostaining of testes using antibodies against markers of undifferentiated spermatogonia. Antibodies against the indicated antigens were used to stain testes of Cdt1-KO2 and Gem-AG transgenic mice. Counterstained with Hoechst 33342 (blue). C: Quantification of cells with undifferentiated spermatogonia marker expression. At least 80 cells with each indicated spermatogonia marker were counted. Bars = 50 μm (A), 20 μm (B).