Skip to main content
. 2014 Feb 18;102(1):97–106. doi: 10.1093/cvr/cvu029

Figure 6.

Figure 6

Contribution of Ser/Thr PPs to the apparent PKA activity in the cytoplasm and the nuclei of ARVMs. (A and B) Effect of concomitant PP1 and PP2A inhibition with CalyA (100 nM) on cytoplasmic (A) and nuclear (B) PKA responses to Iso (100 nM, 15 s) in ARVMs. (C and D) Effect of PP2A inhibition with OA (100 nM) on Iso (100 nM, 15 s)-induced stimulation of PKA in the cytoplasm (C) and the nuclei (D) of ARVMs. (E and F) Effect of PP2B inhibition with CsA (5 µM) on transient β-AR responses in the cytoplasm (E) and the nuclei (F) of ARVMs. Cells were infected with Ad.AKAR3-NES or Ad.AKAR3-NLS for 48 h. OA (black squares), CsA (black squares), or control DMSO (solid lines) were pre-incubated for 1 h at 37°C and then maintained throughout the experiments. CalyA (black squares) or control DMSO (solid lines) was applied 2 min before the 15 s pulse of Iso and then maintained throughout the experiments. Data are from 12 rats. Each symbol in A–F represents the mean ± SEM.