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. 2014 Mar 3;2014:418690. doi: 10.1155/2014/418690

Table 4.

Antioxidant activity (%).

Concentration, mg/L Scavenging activity
25 50 100 200 500
DPPH 29.85 ± 0.86a 31.75 ± 1.41a 40.88 ± 1.15b 47.63 ± 1.93c 71.44 ± 1.04d 229.19 ± 22.34A
ABTS 12.25 ± 0.67a 19.76 ± 0.63b 32.38 ± 0.46c 53.83 ± 0.27d 88.13 ± 0.73e 180.94 ± 3.48A
RP 0.091 ± 0.001a 0.108 ± 0.002b 0.132 ± 0.002c 0.178 ± 0.001d 0.328 ± 0.001e 443.60 ± 4.01B

Assay wavelength: 1,1-diphenyl-2-picrylhydrazyl (DPPH) = 517 nm and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) = 414 nm, and reducing power (RP) = 700 nm.

Butylated hydroxytoluene (BHT) EC50; DPPH: 121.85 ± 0.39 mg/L; ABTS: 93.85 ± 0.43 mg/L; RP: 26.71 ± 0.69 mg/L.

Each value represents mean ± standard deviation (SD), n = 5.

a–eMeans with different superscripts in the row are significantly different at P < 0.05.

AEC50 (mg/L) values were calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate and their data were presented as 50% scavenging activity.

BRP value (EC0.3) was reducing activity calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate.