Table 4.
Antioxidant activity (%).
Concentration, mg/L | Scavenging activity | |||||
---|---|---|---|---|---|---|
25 | 50 | 100 | 200 | 500 | ||
DPPH | 29.85 ± 0.86a | 31.75 ± 1.41a | 40.88 ± 1.15b | 47.63 ± 1.93c | 71.44 ± 1.04d | 229.19 ± 22.34A |
ABTS | 12.25 ± 0.67a | 19.76 ± 0.63b | 32.38 ± 0.46c | 53.83 ± 0.27d | 88.13 ± 0.73e | 180.94 ± 3.48A |
RP | 0.091 ± 0.001a | 0.108 ± 0.002b | 0.132 ± 0.002c | 0.178 ± 0.001d | 0.328 ± 0.001e | 443.60 ± 4.01B |
Assay wavelength: 1,1-diphenyl-2-picrylhydrazyl (DPPH) = 517 nm and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) = 414 nm, and reducing power (RP) = 700 nm.
Butylated hydroxytoluene (BHT) EC50; DPPH: 121.85 ± 0.39 mg/L; ABTS: 93.85 ± 0.43 mg/L; RP: 26.71 ± 0.69 mg/L.
Each value represents mean ± standard deviation (SD), n = 5.
a–eMeans with different superscripts in the row are significantly different at P < 0.05.
AEC50 (mg/L) values were calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate and their data were presented as 50% scavenging activity.
BRP value (EC0.3) was reducing activity calculated from the calibration curves using five different concentrations (25–500 mg/L) in quintuplicate.