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Lentiviral shRNA knockdown of the AAAS gene in H295R (A) and SH-SY5Y (B) cells. AAAS mRNA expression was quantified using real-time qPCR and normalized to GAPDH (n = 3). Control represents transfection with scrambled shRNA. Reduced expression of the protein ALADIN is seen in AAAS-knockdown (AAAS-KD) H295R (C) and SH-SY5Y (D) cells. Data represents mean ± SD. **, P < .01; ***, P < .001.
