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. 2013 Jul 3;154(9):3209–3218. doi: 10.1210/en.2013-1241

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Copyright © 2013 by The Endocrine Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — StAR and P450c11β (CYP11B1) expression are reduced in AAAS-KD H295R cells with a subsequent reduction in cortisol production. Immunoblot analysis (A) and densitometric analysis showing a reduction in StAR (B) (n = 4) and P450c11β protein expression (C) in AAAS-KD H295R cells compared with controls (n = 3), with no effect on P450scc levels (D) (n = 4). E, There is a significant reduction in StAR mRNA levels in AAAS-KD cells compared with controls, and hydrogen peroxide treatment of controls reduces levels to that seen with ALADIN deficiency (n = 3). F, There is a significant reduction in cortisol production by the untreated AAAS-KD H295R cells compared with controls (n = 9), with partial improvement after NAC treatment (n = 3). Data represent mean ± SD. *, P < .05; **, P < .01; ***, P < .001.