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. 2014 Mar 3;2014:927190. doi: 10.1155/2014/927190

Figure 1.

Figure 1

Recombinant DQ8 and DQ2 proteins with gliadin epitopes stimulate T cell clones. (a) Diagrams of the constructs used to produce recombinant protein for deamidated α-gliadin p1E, p9E/DQ8, and α-I gliadin/DQ2. The amino acid sequence of the α-gliadin peptide in DQ8 is QQYPSGEGSFQPSQENPQ and the α-I gliadin peptide (QLQPFPQPELPY) with DQ2. Thrombin, TEV, and PreScission are protease cleavage sites incorporated into the protein constructs. (b) T cell clones restricted to either DQ8 or DQ2 produce IL-2 in response to the deamidated α-gliadin/DQ8 or α-I gliadin/DQ2 recombinant protein, respectively. (c) The DQ2 and (d) DQ8 T cell responses can be blocked in a dose dependent manner with a monoclonal DQ antibody.