Figure 7.

Antileishmanial activity of the crude venom of and PLA₂s from B. mattogrossensis. The promastigote forms of L. amazonensis were plated with 1 × 10⁵ cells/well. Then, different concentrations of crude venom and isolated PLA₂s were added to each well. The experiment was done in a 48 h period, with the (a) crude venom and the PLA₂ enzymes, (b) BmatTX-I, and (c) BmatTX-III. MTT was added and after the incubation period at 33°C, the formazan crystal formed was dissolved in SDS. Readings were done in a spectrophotometer at 570 nm. Each bar represents the average +/− SD of the three independent experimental groups, sixfold total. *P < 0.05 compared to the control.