Table 1.
Effecta | Activity |
---|---|
Phospholipase activityb (U/mg) | 1,864.05 |
Proteolytic activityc (U/10 μg) | 3.0 ± 0.1 |
Hemorrhagic halod (cm2) | 3.33 ± 0.05 |
Coagulation activitye (MCD, μg) | 0.325 |
aAll experiments were carried out in triplicate. bActivity using NOB stained substrate. cOne enzymatic unit (U) was defined as the quantity of enzyme needed to increase the absorbance by 0.05 UA/440 nm. dValues 3 h after incubation with crude venom of B. mattogrossensis (50 μg). eMCD: the minimum coagulation dose was the dose capable of coagulating 200 μL of citrated plasma in less than a minute.