FIG 4 .

Effect of ZapE inactivation on K-12 shape upon FtsZ-GFP and FtsZ expression modulation. (A) Localization of FtsZ-GFP (pDSW230) in K-12 and K12::ΔzapE strains grown in rich media (LB) at 37°C or 42°C in the absence of IPTG until an OD₆₀₀ = 0.5 was reached. Bars are 5 µm. (B) Localization of FtsZ-GFP (pDSW230) in K-12 and K12::ΔzapE strains grown in minimum media (M9) at 37°C in the absence of IPTG until an OD₆₀₀ = 0.5 was reached. Bars are 2 µm. (C) FtsZ-GFP (pDSW230) localization in K-12 and K12::ΔzapE strains during the stationary phase performed in LB rich media at 37°C or 42°C. These observations are representative of the results of at least three independent experiments. Bars are 1 µm. (D) Effect of FtsZ-H₆ (WM971) overexpression on K-12 and K12::ΔzapE shape. Bacteria were grown in LB at 37°C in the presence of the indicated concentrations of IPTG until an OD₆₀₀ = 0.5 was reached. These observations are representative of the results of three independent experiments. Bars are 10 µm.