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. 2014 Jan 27;15(2):1946–1961. doi: 10.3390/ijms15021946

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Heterologous expression of the BmCDA7 gene in yeast and bacteria. (A) The SDS-PAGE analysis of the heterologous expression for the BmCDA7; (B) The western blotting analysis of the heterologous expression for the BmCDA7. Arrow represents the targeted protein. Lane M: Protein marker; Lane 1: The supernatant of the pPIC9K-GS115 induced by 1% methanol for 96 h; Lane 2: The supernatant of the yeast containing recombinant pPIC9K-BmCDA7 plasmid induced by 1% methanol for 96 h; Lane 3: Purified fusion proteins from E. coli with His-tag.