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The temporal expression profiles of BmCDA7. To determine the temporal specificity of expression, three whole silkworm bodies (except for the midgut contents) were collected at the times of the first-fourth instar larvae, middle-stage silkworm and molting silkworm. Total RNA was extracted and was used as template for first-strand cDNA synthesis. Bmactin 3 transcript with the same cDNA template served as an internal control.
