Figure 1.

Isolation and identification of pMSCs. (A) Isolation of pMSCs by the method of tissue culture. Some cells appeared around tissues 10 days after culture. The third generation pMSCs had fibroblast-like morphology visualized by phase-contrast microscopy (100× magnification); (B) Flow cytometry assay of pMSCs: most of pMSCs were CD73, CD90 and CD105 positive; CD34, CD45 and HLA-DR negative; and (C) Differentiation ability of pMSCs: pMSCs can differentiate into cartilage cells and endothelial cells (400× magnification).