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. 2014 Jan 29;15(2):2172–2190. doi: 10.3390/ijms15022172

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Int6/eIF3e inhibition halts GBM cell cycle. (A) Quantification of cell cycle distribution of GBM cells using flow cytometry and PI staining. Int6 knockdown (siInt6) induces decreased cell number in S phase and increased cell number in G1 phase compared to control cells (siScr); (B) Quantification of GBM cells in G0 phase using flow cytometry and Ki67/PI staining. Int6 knockdown significantly increases the number of cells in G0 phase (* p < 0.05, *** p < 0.001 compared with the negative control, siScr, n = 4).