Figure 6.

Int6 gene silencing downregulates the Hypoxia Inducible Factors expression without affecting global cell translation. (A,B) De novo protein synthesis in GBM cell lines (72 h after siScr or siInt6 transfection) was assessed by ³⁵S Methionine metabolic labeling. Proteins were resolved by SDS-polyacrylamide gel, autoradiographed (A) and stained with Coomassie (B); (C,D) Western blot analysis of HIF-2α (C) and HIF-1α (D) expression in human GBM cells transfected or not with siInt6 showing decreased HIF expression when Int6/EIF3E is knockdowned, n = 5; (E) Graphs representing HIF target gene (VEGF, PDGF-β, Dll4, αV integrin) expression in control or Int6 silenced GBM cells, assessed by qRT-PCR. Int6 inhibition significantly reduces HIF target gene expression. (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 4).