Figure 1.

Schematic representation of the pathway for N-linked glycoprotein biosynthesis. In this process, a Man₅GlcNAc₂-PP-dolichol lipid-linked precursor intermediate is assembled. This intermediate is then extended in the lumen of the ER until Glc₃Man₅GlcNAc₂-PP-dolichol lipid-linked precursor is completed. The Glc₃Man₅GlcNAc₂ oligosaccharide is then transferred onto the target nascent protein to form a protein precursor. This protein precursor is then deglucosylated/reglycosylated to ensure quality control of the neosynthetized protein. The proteins involved are listed in the yellow squares and annotated in Table S1.