Table 3.
Existence of the conserved domain of Porphyridium sp. proteins involved in ER N-glycosylation. Separately, sequence detection of conserved domains was done directly (Blast2Go versus Interpro scan and then added GO’s (Gene ontology).
| Abbreviation | Definition | Domain Identification | Name | E Value |
|---|---|---|---|---|
| DK | Dolichol kinase | PTHR13205:SF8 | transmembrane protein 15 | 6.70E–29 |
| ALG7 | UDP-N-acetylglucosamine—dolichyl-phosphate N-acetylglucosaminephosphotransferase | PF00953 | Glyco_transf_4 | 1.20E–49 |
| ALG13 | UDP-GlcNAc:dolichyl-pyrophosphoryl-GlcNAc GlcNAc transferase | PF04101 | Glyco_transf_28_C | 1.10E–26 |
| ALG14 | UDP-GlcNAc:dolichyl-pyrophosphoryl-GlcNAc GlcNAc transferase | PF08660 | Alg14 | 2.00E–72 |
| ALG1 | chitobiosyldiphosphodolichol beta-mannosyltransferase | PF00534 | Glyco_transf_1 | 9.80E–11 |
| ALG2 | glycolipid 3-alpha-mannosyltransferase | PF00534 | Glyco_transf_1 | 5.40E–32 |
| ALG11 | GDP-mannose:glycolipid 1,2-alpha-d-mannosyltransferase | PF00534 | Glyco_transf_1 | 8.30E–23 |
| ALG3 | Dolichol phosphomannose-oligosaccharide-lipid mannosyltransferase | PF05208 | ALG3 | 4.10E–143 |
| ALG9 | Dolichol phosphomannose-oligosaccharide-lipid mannosyltransferase | PF03901 | Glyco_transf_22 | 8.50E–97 |
| ALG12 | Alpha-1,6-mannosyltransferase | PF03901 | Glyco_transf_22 | 1.30E–19 |
| ALG6 | Alpha-1,3-glucosyltransferase | PF03155 | ALG6_ALG8 | 1.90E–107 |
| ALG8 | Alpha-1,3-glucosyltransferase | PF03155 | ALG6_ALG8 | 3.80E–85 |
| ALG10 | Alpha-1,2 glucosyltransferase | PF04922 | DIE2_ALG10 | 1.30E–15 |
| ALG5 | Dolichyl-phosphate beta-glucosyltransferase | PF00535 | glyco_transf_2 | 7.50E–23 |
| DPM1 | Dolichol-phosphate mannosyltransferase | PF00535 | Glyco_transf_2 | 8.30E–34 |
| Dol-P-Glc phosphodiesterase | - | - | - | |
| STT3 | PF02516 | STT3 | 1.40E–142 | |
| PF02517 | STT3 | 3.30E–133 | ||
| OST1 | PF04597 | Ribophorin I | 2.50E–19 | |
| OST 2 | PF02109 | DAD | 1.20E–41 | |
| OST 3/6 | Dolichyldiphosphoryloligosaccharide-protein (OST) | - | - | - |
| OST 4 | PF10215 | Ost4 | 7.80E–06 | |
| OST 5 | - | - | - | |
| WBP1 | PF03345 | DDOST_48kD | 1.20E–87 | |
| SWP1 | - | - | - | |
| RFT1 | Flippase | PF04506 | Rft-1 | 9.20E–11 |
| GCS1 | Mannosyl-oligosaccharide glucosidase I | PF03200 | Glyco_Hydro 63 | 1.50E–87 |
| GANAB | Alpha 1,3-glucosidase II | PF01055 | Glyco_Hydro 31 | 5.90E–282 |
| GANABb | Alpha 1,3-glucosidase II,beta subunit | PTHR12630:SF1 | Glucosidase II b subunit | 2.70E–28 |
| MAN1a | Mannosyl-oligosaccharide alpha-1,2-mannosidase | PTHR11742 | Mannosyl-oligosaccharide alfha-1,2-mannosidase related | 3.80E–131 |
| MAN1b | 7.80E–95 | |||
| MAN1c | 1.40E–98 | |||
| UGGT | UDP-glucose:glycoprotein glucosyltransferase | PTHR11226 | UDP—glucose glycoprotein: glucosyltransferase | 2.30E–168 |
| CALNEX | Calnexin | PF00262 | Calreticulin | 1.00E–147 |
| CALRET | Calreticulin | PF00262 | Calreticulin | 2.90E–63 |