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. 2014 Feb 11;15(2):2431–2453. doi: 10.3390/ijms15022431

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Genetic studies of pfptpa and pfpp2a. (a,b) Gene-targeting construct for gene disruption by single homologous recombination using the pCAM-BSD, and the locus resulting from integration of the knockout pfptpa (a) or pfpp2a (b) construct. (c,d) Analysis of pCAM-BSD-pfptpa (c) and pCAM-BSD-pfpp2a (d) transfected 3D7 culture by PCR; lanes 1–3 correspond to DNA extracted from transfected parasites; lanes 4–6 correspond to DNA extracted from wild type parasites. Lanes 1 and 4 represent the detection of the full length wild type locus (PCR with p9-p8 and p31-p30 respectively); lanes 2 and 5 represent the detection of episomal DNA (PCR with p10 and p11); and lanes 3 and 6 represent the detection of integration of the insert (PCR with p9-p11 and p31-p11 respectively).