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. 2014 Feb 13;15(2):2517–2537. doi: 10.3390/ijms15022517

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Stable expression of MYB44-REP in transgenic Arabidopsis plants. (A) Documentation of overexpression. Eight-day-old seedlings of Col-0 and MYB44-REP transgenic lines were separated by SDS-PAGE. Transgene expression was visualized by immunodetection, using rabbit anti-myc and IRDye800CW-coupled donkey-anti-rabbit (NEB, Frankfurt, Germany) as primary and secondary antibodies, respectively. Strong transgene expression can be seen in five lines; and (B) MYB44-REP overexpression in Arabidopsis does not interfere with normal development; (B1): 10-day-old seedlings grown on standard medium; and (B2): Aerial views of rosettes of 5-week-old plants of wild-type and MYB44-REP overexpressing plants. Images of one representative line, which was phenotypically indistinguishable from other MYB44-REP lines, are shown.