Table 1.
Primers for cloning of promoters.
| Primer name | Sequence |
|---|---|
| bu7SK-F | 5′-TCTAGAGAGACAGACCTGGCTCCAC-3′ a |
| bu7SK-R | 5′-GAGCTCCACATCCGAGACACTCTGC-3′ b |
| buU6-F | 5′-ATCGATGAGCATTCAGTCCGGCAG-3′ c |
| buU6-R | 5′-CTCGAGGCACGGTAAACATGGCTTC-3′ d |
| h7SK-F | 5′-TCTAGACTGCAGTATTTAGCATGCCCC-3′ a |
| h7SK-R | 5′-GAGCTCGAGGTACCCAGGCGGC-3′ b |
| hU6-F | 5′-ATCGATCCCCCGAGTCCAACAC-3′ c |
| hU6-R | 5′-CTCGAGGTGTTTCGTTCTTTCCACAAG-3′ d |
| bo7SK-F | 5′-TCTAGAGAGCGTGAGAGACTCGGAGC-3′ a |
| bo7SK-R | 5′-GAGCTCCACCATGGTAGTTCGCGCAG-3′ b |
| boU6-F | 5′-ATCGATAAGAGGCTCCTGAGCAACG-3′ c |
| boU6-R | 5′-CTCGAGCATTTACCCCCCACAGAATATATAAG-3′ d |
a
The underlined sequence indicates XbaI restriction endonuclease site;
b
The underlined sequence indicates SacI restriction endonuclease site;
c
The underlined sequence indicates ClaI restriction endonuclease site;
d
The underlined sequence indicates XhoI restriction endonuclease site.