Figure 6.

The caspase inhibitor Z-VAD-FMK attenuates kaempferol-induced apoptosis in HT-29 cells. Cells were plated in 24-well plates and treated with 50 μmol/L Z-VAD-FMK for 1 h. Cells were then treated with 0 or 60 μmol/L kaempferol with or without 50 μmol/L Z-VAD-FMK for 48 h. Cells were trypsinized, loaded with Annexin V and 7-AAD, and analyzed by flow cytometry. The percentages of early apoptotic cells were calculated. Each bar represents the mean ± SEM (n = 3). Means without the same letter (a, b or c) are significantly different (p < 0.05).