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. 2014 Feb 18;15(2):2738–2760. doi: 10.3390/ijms15022738

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 12. — RT-qPCR analysis of ScChi and the immunity related marker genes in the Nicotiana benthamiana leaves 24 h after transiently expressing pCAMBIA 1301-ScChi and the empty vector. (A) The transcript analysis of ScChi; (B) the transcript analysis of the immunity related marker genes, including the hypersensitive response marker genes, NtHSR201, NtHSR203 and NtHSR515, the salicylic acid related gene, NtNPR1, the jasmonate associated genes, NtPR-1a/c, NtPR2 and NtPR3, and the ethylene synthesis depended genes, NtEFE26 and NtAccdeaminase. NtEF1-α was used to normalize the transcript levels. Mock: the Agrobacterium strain carrying 35S::00. All data points are the means ± SE (n = 3). Different lowercase letters indicate a significant difference, as determined by the least-significant difference test (p-value < 0.05).

Figure 12. — RT-qPCR analysis of ScChi and the immunity related marker genes in the Nicotiana benthamiana leaves 24 h after transiently expressing pCAMBIA 1301-ScChi and the empty vector. (A) The transcript analysis of ScChi; (B) the transcript analysis of the immunity related marker genes, including the hypersensitive response marker genes, NtHSR201, NtHSR203 and NtHSR515, the salicylic acid related gene, NtNPR1, the jasmonate associated genes, NtPR-1a/c, NtPR2 and NtPR3, and the ethylene synthesis depended genes, NtEFE26 and NtAccdeaminase. NtEF1-α was used to normalize the transcript levels. Mock: the Agrobacterium strain carrying 35S::00. All data points are the means ± SE (n = 3). Different lowercase letters indicate a significant difference, as determined by the least-significant difference test (p-value < 0.05).