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Nucleotide acid sequences obtained by real-time (RT)-PCR and the deduced amino acid sequences of ScChi. The deduced amino acid sequences are shown as a one-letter code under the cDNA sequences. The underline indicates the catalytic domains (DXXDXDXXXE) of the glycosyl hydrolase family 18. The eight red squares of Tyr10 (Y10), Phe37 (F37), Asp140 (D140), Asp142 (D142), Phe144 (F144), Gln195 (Q195), Phe259 (F259) and Trp261 (W261) show putative carbohydrate binding sites.
