Figure 7.
Spot assays of Rosetta + pET 32a-ScChi (a) and Rosetta + pET 32a (control) (b) on Luria-Bertani (LB) plates with NaCl, CuCl2, CdCl2 and ZnSO4. Isopropyl β-d-thiogalactoside (IPTG) was added to the cultures of Rosetta + pET 32a-ScChi and Rosetta + pET 32a to induce the expression of recombinant protein. The cultures were adjusted to OD600 = 0.6. Ten microliters from 10−3 (left side of the red line on the plate) to 10−4 (right side of the red line on the plate) dilutions were spotted onto LB plates without any supplement (CK) or with NaCl (250, 500 and 750 mM), CuCl2 (250, 500 and 750 μM), CdCl2 (250, 500 and 750 μM) and ZnSO4 (250, 500 and 750 μM), respectively. NaCl, sodium chloride; CuCl2, copper chloride; CdCl2, cadmium chloride; ZnSO4, zinc sulfate.