Table 2.

Comparison of typical efficiency of genome editing.

Host bacteria	Method used	Efficiency	Reference
E. coli	λ-red recombinase method, double stranded DNA	103 to 104 recombinants per 108 viable cells a	[92]
E. coli	λ-red recombinase method, single stranded DNA	~107 recombinants per 108 viable cells	[92]
E. coli	λ-red recombinase method, single stranded DNA	25% b	[25]
L. reuteri	λ-red recombinase method, single stranded DNA	0.4%–19% b	[31]
E. coli	mobile group II introns	1%–80% b	[43]
C. thermocellum	mobile group II introns	67%–100% b	[49]
S. aureus	mobile group II introns	37%–100% b	[51]
S. pneumoniae	CRISPR-Cas9 system	100% b	[74]
E. coli	CRISPR-Cas9 system	65% b	[74]

^aReplacing the galK gene with a drug cassette;

^bEfficiency is calculated as percentage of successful recombination per appeared colonies without any selection pressure.