Table 3.
Genes silenced with HPasRNAs in E. coli.
| Gene name | Gene product | Silencing efficacy a | Observed phenotypes upon expression of HPasRNAs |
|---|---|---|---|
| lacZ | β-galactosidase | 88% [93] | – |
| ackA | Acetate kinase | 78% [94] | Reduced acetate production, no growth on minimal acetate media [95] |
| aceE | Pyruvate dehydrogenase component | Acetate auxotroph, accumulation of pyruvate [93] | |
| ftsZ | Tubulin-like protein | Severe growth (essential gene), elongated cell [105] | |
| fusA | Elongation factor G | Severe growth (essential gene), sensitization 12-fold to fusidic acid [106] | |
| (Many growth essential genes) | Construction of a shotgun genomic library expressing HPasRNAs, identification of growth essential genes [106] | ||
| mutT | Protein for maintaining DNA replication fidelity | >90% [106] | Protein level control in a stepwise fashion by changing concentration of expression inducer (IPTG) [107] |
| mutS, mutD, ndk (triple silencing) | Proteins for maintaining DNA replication fidelity | Increased mutation rate by 2000-fold over wild-type cells [94] |
a
Evaluated as reduced protein activity upon HPasRNA expression.