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. 2014 Feb 20;15(2):2946–2958. doi: 10.3390/ijms15022946

Figure 1.

Figure 1.

HuR regulates the SIRT1-ΔExon8 mRNA levels in 293T cells and U251 cells. (A) The changes of HuR protein levels after HuR overexpression or silencing in 293T cells; (B) PCR products were separated in 1% agarose gels, the arrows point at the location of SIRT1-FL and SIRT1-ΔExon8 corresponding to the 200 and 111 bp of the left mark, respectively; GAPDH was used as the loading control, the signal ratio was calculated as the ratio of signal for the SIRT-FL or SIRT1-ΔExon8 band and the GAPDH band; (C–F) Splice-variant-specific quantitative Real-Time PCR (qRT-PCR) of SIRT1-FL and SIRT1-ΔExon8 were shown in 293T cells and U251 cells. The levels of SIRT1-ΔExon8 mRNA were increased after HuR over-expression to 2.17 fold in 293T cells (C) and 3.29 fold in U251 cells (E). The interference of HuR resulted in the notable reduction of SIRT1-ΔExon8 with 62% in 293T cells (D) and 28% in U251 cells (F); * p < 0.05 compared to corresponding control.