Figure 2.

HuR and TIA1/TIAL1 regulate alternative splicing of SIRT1 exon 8 of SIRT1 minigene; (A) Schematic structure of SIRT1 minigene, shaded boxes are alternatively spliceable exons; (B) The arrows point at the location of the primers of SIRT1; (C) HuR caused a significant increase in exon-exclusion for SIRT1 RNAs; (D) TIA1 induced SIRT1 minigene exon 8 inclusion. The splicing band patterns were quantified using Image J software [38] and the results showed as below. The results obtained from three independent experiments. ^* p < 0.05 compared to co-transfect empty plasmid and SIRT1 minigene.