Figure 3.

Delay in cell growth caused by overexpression of miR-574-3p in A549 cells. (A) A549 cells were transfected with 10 or 20 nM synthetic miR-574-3p or miR-574-3p antagomirs. Cells transfected with the synthetic miR-574-3p or miR-574-3p antagomirs were incubated for 24 h and then re-plated into the wells of a device that allows real-time measurement of cell growth (calculated as the cell index) at 1-h intervals. Each experiment was conducted four times; (B) Cell growth was assessed using the cell index at 36 h. * p < 0.05 vs. mock-transfected cells.