Figure 4.

Attenuation of X-ray-induced growth delay in A549 cells through suppression of miR-574-3p. (A) A549 cells were transfected with 20 nM miR-574-3p antagomirs or synthetic miR-574-3p. Cells transfected with the miR-574-3p antagomirs or synthetic miR-574-3p were incubated for 24 h and then irradiated with 5-Gy of X-ray. X-irradiated cells were seeded on 35-mm dishes at a density of 67,000 cells per dish. Cells were counted using a hemocytometer at 24, 36, 48, or 72 h after irradiation. Each experiment was conducted three times; (B) Cell growth was assessed using the cell number at 36 h. * Statistical difference with p < 0.05 was considered significant.