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. 2014 Feb 21;15(2):3204–3219. doi: 10.3390/ijms15023204

Figure 1.

Figure 1.

Cell growth and analysis of extracellular protease activities. (A) Growth kinetics of S. solfataricus at 80 °C in YS (yeast extract and sucrose; white circle), TYS (tryptone, yeast extract and sucrose; triangle) or PYS (peptone, yeast extract and sucrose; black circle) supplemented media. The arrows indicate the stages at which the cells were harvested for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. (insert): Lane 1, molecular weight markers; Lane 2, extracellular proteins from PYS cultures; Lane 3, extracellular proteins from TYS cultures; and Lane 4, extracellular proteins from YS cultures; (B) Gelatin zymogram under non-denaturing conditions of extracellular proteins from TYS, PYS and YS cultures. Lanes 1–2, 9 U of total protease activity released into PYS and TYS media, respectively; Lanes 3–5, 7.0 μg of total extracellular protein from PYS, TYS and YS cultures, respectively; and Lanes 6–7, control samples of concentrated PYS or TYS culture broth, respectively; and (C) Analysis of the extracellular TYS-proteome by SDS-gelatin zymogram. Lane 1, extracellular proteins from TYS culture pre-warmed at 60 °C for 1 h; and Lane 2, extracellular proteins from TYS culture not thermally denatured; and Lane 3, molecular weight markers.