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. 2014 Feb 21;15(2):3319–3335. doi: 10.3390/ijms15023319

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© 2014 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 5. — Western blotting and quantification. (A) Western blotting analysis of Thellungiella salsuginea glutathione peroxidase (TsGPX) in leaves or roots of T. salsuginea before (0) and at 6-, 12-, and 24-h after NaCl or PEG 6000 treatment. Anti-OsGAPDH antibody was used as a control for loading error; (B) Bar graph for the quantitative comparison among TsGPX protein expression levels during NaCl treatment; (C) Bar graph for the quantitative comparison among TsGPX protein expression levels during PEG 6000 treatment. TsGPX- and OsGAPDH-specific bands were quantified by ImageJ software and TsGPX protein expression levels were normalized to GAPDH values. Mean values ± SD of 3 independent experiments are shown. Asterisks denote significant up-regulation (ratio > 2.0) or down-regulation (ratio < 0.5).