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. 2014 Mar 19;4:4413. doi: 10.1038/srep04413

Figure 4. Tamoxifen-induced cell death with siRNA transfection.

Figure 4

(A) Knockdown of SNF1LK, TRAP1, JAK2, SOCS2, and FOSB was enabled by siRNA transfection as described in Materials and Methods. At 48 h post-transfection, cells were treated with tamoxifen (1 μM) for 24 h, and then cell viability was measured using the WST-1 assay (mean ± SEM; Tukey's post hoc test was applied to significant group effects in ANOVA, P < 0.0001; ***P < 0.001, compared with non-treated control). (B) Cell viability was evaluated by flow cytometric analysis after TMRE staining.