Table 2. Steady-state activity of the covalent complex (PCXL) compared with C128A (wild-type CCP) and the V197C/C128A mutant at pH 6.
|
Kcat, sec-1
|
||||
|---|---|---|---|---|
| 20 mM potassium phosphate
|
200 mM potassium phosphate
|
|||
| Enzyme | Horse cyt. c | Yeast cyt. c | Horse cyt. c | Yeast cyt. c |
| WT CCP | 850.3 ± 51.0 (100) | 219.1 ± 11.8 (100) | 175.1 ± 9.5 (100) | 1361.8 ± 57.4 (100) |
| V197C/C128A | 803.4 ± 17.0 (95) | 240.9 ± 7.3 (109) | 166.8 ± 7.2 (95) | 1167.2 ± 17.7 (86) |
| PCXL | 20.7 ± 2.0 (2.4) | 15.7 ± 0.9 (7.2) | 4.2 ± 0.09 (2.4) | 76.2 ± 4.2 (5.6) |
The values in parentheses are percent of wild-type activity.