Table 2. Thiosulfate sulfurtransferase activity and in vitro formation of MPT by MOCS3-RLD and variants.

Protein	Formed thiocyanate,* nmol/min/mg	MPT formation,† %
MOCS3-RLD	44.2 ± 2.3	100
C316A	45.1 ± 0.9	102.3 ± 3.5
C324A	46.6 ± 1.3	99.25 ± 8.3
C365A	39.3 ± 3.0	106.5 ± 13.2
C412A	1.3 ± 0.9	ND

ND, not detected.

^*Thiocyanate (complexed with iron) was quantitated as A₄₆₀ (26).

^†MPT was quantitated as form A (18). The amount of MPT formation of wild-type MOCS3-RLD was set to 100%.