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. 2004 Apr 12;101(16):6021–6026. doi: 10.1073/pnas.0401590101

Fig. 3.

Fig. 3.

Med contributes to salE/Pv enhancer expression in certain regions of the wing blade. (A and B) EMSA experiments using radioactive oligonucleotides and bacterially expressed Med protein at increasing concentrations. The sequences of the oligonucleotides used as probes are indicated under each panel. The sequence consensus for Mad–Med binding is shaded in gray. The mutated nucleotides are indicated in lowercase. Open arrows indicate Med binding, whereas open arrowheads indicate the free probe. (A) Med binds to oligonucleotides 274, 413, and 429. (B) Mutated oligonucleotides 274aaa, 413t5, and 429t6 do not bind Med. (C) Expression of β-gal in third-instar discs driven by mutant salE/Pv enhancers. (D) Measurements of the areas of β-gal expression in relation to endogenous sal expression. The antero/posterior extension of the β-gal domain in the different mutants does not change in respect to the salE/Pv control.