Figure 6.
Effect of SP on MMP3 protein level. Analysis of MMP3 after 12 hours of incubation with SP (10−7 M), without SP (control), and with SP and the NK-1 R blocker (10−6 M), as measured by normalized band intensity. MMP3 was significantly increased after incubation with SP (***p<0.001, one-way ANOVA, followed by Bonferroni post-hoc test) and this increase was much less obvious in the presence of the NK-1 R antagonist (***p<0.001, one-way ANOVA, followed by Bonferroni post-hoc test) when both are compared to the control. As well, the NK-1 R antagonist resulted in significant decreased in MMP3 protein level relative to treatment with SP (***p<0.001, one-way ANOVA, followed by Bonferroni post-hoc test).