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. 2014 Mar 6;5:3408. doi: 10.1038/ncomms4408

Figure 4. Effects of CBR703 and substitutions at the BH-β subunit interface on pausing at the regulatory pause site.

Figure 4

(a) Transcript elongation on pIA171 template by wild-type, β′F773V and β′H777A RNAPs in the absence (left panels) or in the presence (right panels) of 100 μM CBR703. (b) Quantification of the fraction of RNA at the hisP (transcript position U71) from the data shown in a and Supplementary Fig. 3. Fraction refers to the ratio of the paused RNA to the sum of all products resulting from elongation of TEC halted at A29. Error bars are s.d. of three independent experiments.