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. 2014 Mar 18;5:3492. doi: 10.1038/ncomms4492

Figure 8. Blockade of TLR3 binding to RNA ameliorates radiation-induced GIS.

Figure 8

(a) Kaplan–Meier survival analyses of mice treated with TLR3/dsRNA complex inhibitor before TBI and isologous BMT. n=6; **P<0.01 (log-rank test). (b,c) Severity of diarrhea (b) and body weight loss (c) of non-treated and TLR3/dsRNA complex inhibitor-pretreated mice. n=6; results are means±s.e.m. *P<0.05; **P<0.01 (unpaired two-tailed Student’s t-test). (d) TUNEL-staining of the small intestinal crypts of non-treated and TLR3/dsRNA complex inhibitor-pretreated mice at 6 h. TUNEL-stained epithelium shows green fluorescence. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. The right-hand panel shows numbers of TUNEL-positive cells. n=5; results are means±s.e.m. *P<0.05 (unpaired two-tailed Student’s t-test). (e) H&E staining of the small intestinal crypts of non-treated and TLR3/dsRNA complex inhibitor-pretreated mice on day 3. Scale bar: 100 μm. Asterisks denote microcolonies. Right panel shows the numbers of microcolonies. n=5; results are means±s.e.m. **P<0.01 (unpaired two-tailed Student’s t-test). Results are representative of three independent experiments.