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RBPMS immunoreactivity was mainly expressed in medium and large cells in the rat GCL. Rat whole-mounted retinas immunostained with RBPMS antibodies and counterstained with DRAQ5 or NeuroTrace. DRAQ5 stained all nuclei, and NeuroTrace stained RGC and displaced amacrine cell bodies. A, D: RBPMS. B: DRAQ5. E: NeuroTrace. C, F: Merged images. Plane of focus in GCL for all images. z-step = 0.51 μm. 13 optical sections were compressed for viewing. RBPMS antibody GP15029. Scale bar = 20 um.
