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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Pathog Dis. 2013 Nov 11;70(2):189–193. doi: 10.1111/2049-632X.12104

Fig. 2.

Fig. 2

Glycogen staining and qRT-PCR analysis of C. muridarum plasmid gene mutants. (A) Phase microscopy and iodine staining of McCoy cells infected with Cm, CmP−, Cmp+ and CmpΔpgp4. Cells were infected with a MOI of 0.25 and examined at 30 h post-infection (magnification 200×). Arrows indicate inclusions. (A, a) Cm. (B, b) CmP−. (C, c) CmP+. (D, d) CmpΔpgp4. The inclusions of CmP− and CmpΔpgp4 exhibit a donut-like appearance and exhibited reduced glycogen staining. (B) glgA and pgp3 transcript levels in C. muridarum strains. The differences in glgA expression is significantly different (p<0.05) in CmP− vs Cm and CmpΔpgp4 vs Cmp+ comparisons. The differences in pgp3 expression is significantly different (p<0.05) in CmP− vs Cm and CmpΔpgp4 vs Cmp+ comparisons.