Fig. 9.

Ethanlol (EtOH)-thiamine deficiency (TD) reduces excitatory amino acid transporter (EAAT1) immunoreactivity in thalamus (THAL), but not in entorhinal cortex (ENT). C57BL/6 mice were treated with vehicle and EtOH+TD10 as described in Materials and Methods. Animals were sacrificed 24 hours following the last dose of EtOH treatment (5 g/kg, i.g. daily for 10 days). Brain sections were immunostained with astrocytic glutamate transporter EAAT1 antibody. EAAT1 immunoreactivity did not show any change in ENT between vehicle and EtOH+TD10 groups. However, EtOH+TD10 treatment significantly decreased EAAT1 + IR cells in THAL, compared with vehicle controls. Scale bar = 50 μm.