Fig. 1.
DC presentation of MHC class I molecule-restricted viral epitopes from HIV-infected apoptotic CD4+ T cells. (A) DC were incubated with apoptotic cells purified from irradiated H9 or H9-HIV cells at an H9:DC ratio of 3:1, then with LPS, and tested by using a CD8+ T cell line specific for RT476–84 epitope at a DC:effector ratio of 1:3. As control, DC were incubated with 1 μM peptide. (B) The test was carried out by using DC expressing or not the class I MHC restriction molecule HLA-A3. (C) DC:H9 coculture was performed in the presence or not of AZT or in transwells (TW). (D) DC were cultured with irradiated 8E5 cells expressing viral proteins after activation (8E5act) in the absence of LPS and tested as before. Data are representative of at least three experiments each, except for B and C (two experiments).