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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2014 Jan 13;20(4):463–473. doi: 10.1016/j.bbmt.2014.01.006

Figure 2. Picomolar concentrations of IL-2 induce phospho-STAT5 expression in pre-activated NK cells.

Figure 2

Purified NK cells were pre-activated with LD15 (control), IL-12+IL-18, or IL-15+IL-18, washed extensively, and re-plated in cytokine-free media for 2 days to allow recovery to a resting state. IL-2 was added at the indicated concentrations for 15 minutes and levels of phospho-STAT5 were analyzed by intracellular flow cytometry. Shown is one representative donor (A), or summary data of the mean ± SEM percentage pSTAT5 positive CD56dim NK cells from n=3 donors and experiments (B). Pre-activated NK cells respond to 10 and 100 pM IL-2 with enhanced STAT5 phosphorylation over control. Significance was calculated by ANOVA.