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. 2004 Apr 8;101(16):6158–6163. doi: 10.1073/pnas.0401602101

Fig. 5.

Fig. 5.

SH2 domains of Syk and an intact DAP12 ITAM are required for in vitro osteoclast differentiation and function. TRAP+ MNC (A) and the percent resorption (B) of calcium phosphate substrate by syk-/- precursors infected with retrovirus encoding vector alone, wild-type syk, or a SH2-dead mutant (R194A) of syk at indicated M-CSF concentrations. In both conditions, TRAP+ MNC number and percent resorption in the SykWT groups was statistically different (P < 0.01) from vector or SykSH2-Dead, with no difference between vector and SykSH2-Dead groups (P > 0.05). (C) Anti-Syk blot of whole-cell ligands from retrovirally transduced or wild-type OCLs. TRAP+ MNC (D) and percent resorption (E) from DAP12-/-FcRγ-/- precursors infected with virus-encoding vector alone, wild-type DAP12, or ITAM tyrosine mutants (Y65, Y76, or both) of DAP12 cultured in 10 ng/ml M-CSF. (F) Expression of FLAG epitope on cells retrovirally transduced with FLAG-tagged DAP12 or FLAG-tagged DAP12 mutants.