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. 2004 Apr 12;101(16):6188–6193. doi: 10.1073/pnas.0400542101

Fig. 3.

Fig. 3.

Characterization of Ad5-T3Dσ1. Ad5 virions expressing wild-type fiber (Fiberwt), CAR-ablated biotinylated fiber (Fiber-BAP-TR) (10), and Fibtail-T3Dσ1C2H6 were precipitated with trichloroacetic acid. (A) Precipitated particles (4 × 1010 per lane) were resolved by SDS/PAGE and immunoblotted with anti-c-Myc mAb 9E10 or antiserum 1561, which recognizes the N-terminal region of Ad5 fiber. (B) Precipitated particles (1.5 × 1011 per lane) were resolved by SDS/PAGE and stained with Coomassie blue.