Fig. 1.

(A) Ad-induced up-regulation of DC maturation marker CD86. DC were infected with Ad5βgal at 5,000 particles per cell (p/c) or mock infected with viral storage buffer. Flow cytometry histograms show the expression of CD86 on the surface of uninfected and adenoviral-infected DC (staining as described in Materials and Methods). DC in the CD86 High-M2 peak are characterized as the mature DC or High-DC. (B) TNF ELISA showing Ad-induced TNF secretion from DC. As a positive control, DC were exposed to 20 ng/ml LPS. TNF-α secretion was measured 24 h after infection.