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Ad-induced DC maturation is MyD88-independent. DC were incubated with or without anti-TNF antibody and infected with Ad5βgal at 5,000 particles per cell or mock-infected with viral storage buffer. Twenty-four and 48 h after infection, cells were incubated with anti-CD86 antibody. Flow cytometry was used to assess cell surface expression of CD86 as a measure of mean fluorescence intensity (A) or percentage of total DC population in a mature state (B).
