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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Eur J Immunol. 2014 Jan 13;44(3):673–682. doi: 10.1002/eji.201343717

FIGURE 4. miR-107 inhibits IL-23p19 and IL-23 production in BMDCs.

FIGURE 4

(A) BMDCs were treated with the indicated TLR ligands for 24 h and expression of IL-23p19 was analyzed by quantitative PCR. Data are shown as mean ± SD of two samples from one of three experiments performed. *p<0.01 compared with control BMDCs, unpaired, two tailed Student’s t test. (B–D) BMDCs were transfected with miR-107 precursor (pre-miR-107) and cultured with or without LPS stimulation for 24 h. A scrambled microRNA precursor (pre-miR-Ctrl) was used as negative control. (B) Expression of IL-23p19 was analyzed by quantitative PCR. (C, D) Production of (C) IL-23 and (D) other cytokines in supernatants following 24 h culture was determined by ELISA. (B–D) Data are shown as mean ± SD of two samples from one of three experiments performed. *p<0.05 compared with control BMDCs, unpaired, two tailed Student’s t test.